![]() ![]() monocytogenes has been found in irrigation waters and agricultural soils, and can spread to agronomical fresh product risking food safety. One of the prime reasons for listeriosis outbreaks is inadequate hygiene standards and insufficient implementation of standard sanitation operating procedures in the food manufacturing industry. monocytogenes were also recorded, in an instance after detecting this bacterium in urine samples. Patients with chronic renal failure and cirrhosis, as well as those on drugs to reduce gastric acidity, are at greater risk of listeriosis. Invasive listeriosis is responsible for meningitis in immunocompromised people and miscarriage in pregnant women. Listeric infections are often associated with gastrointestinal (GI) listeriosis (non-invasive) in immunocompetent persons or listeriosis (invasive) in immune-compromised individuals. Listeria monocytogenes is a pathogenic facultative, intracytosolic, gram-positive bacterium for humans and several animal species which is responsible for the infection-listeriosis. Keywords: hypothetical protein, in silico characterization, Listeria monocytogenes, PemK/MazF-like toxin, three-dimensional structure, type II toxin-antitoxin system Our aim to annotate the HP Imo088 found in Listeria could offer a chance to understand bacterial pathogenicity and identify a number of potential targets for drug development. Most pathogens that harbor TAS create a crucial risk to human health. The active site of the developed 3D structure was determined by the CASTp server. Following the YASARA energy minimization method, the target protein's 3D structure became more stable. Various quality evaluation tools, such as PROCHECK, ERRAT, Verify 3D, and QMEAN were used to validate the 3D structure. The Alpha Fold 2 Protein Structure Prediction Database was used to determine the three-dimensional (3D) structure of the HP using the template structure of a type II TAS PemK/MazF family toxin protein (DB ID_AFDB: A0A4B9HQB9) with 99.1% sequence identity. Through secondary structure analysis, we found the random coil to be the most frequent. Domain and motif analysis revealed that the target protein is a PemK/MazF-like toxin protein of the type II toxin-antitoxin system (TAS) which was consistent with BLASTp analysis. Various techniques, including CELLO, PSORTb, and SOSUIGramN, identified the candidate protein as cytoplasmic. In this study, a HP Imo0888 (NP_464414.1) from the Listeria monocytogenes EGD-e strain was annotated using several bioinformatics tools. The functions of hypothetical proteins (HP) from various pathogenic bacteria have been successfully annotated using a variety of bioinformatics strategies. For geneticists, biologists, and especially students, however, it's a versatile, compatible lab-grade research tool.The gram-positive bacterium Listeria monocytogenes is an important foodborne intracellular pathogen that is widespread in the environment. It's easy to use, but it requires specific knowledge to make proper use of it. Sequence Viewer is freeware that works with all recent versions of Windows up to Vista. Sophisticated reporting features simplify publishing data in papers. Sequence Viewer's standout feature is its views, which clearly depict the results of complex genetic sequences, statistics, and enzyme analysis in colorful graphics that can be exported into a staggering array of formats. There's a main pane view and left-hand side panes with tree views of the Navigation Area and Toolbox. The program's main interface features the familiar businesslike Windows layout, with a toolbar that lists common menu items such as File, Edit, and Help as well as program-specific menus like Workspace. ![]() It offers a continuously evolving lab-grade application in a compact, easy-to-use format that can access many integrated research tools and databases. It also carries over the premium utilities' sophisticated data management and exporting capabilities as well as compatibility with a wide range of platforms and file formats. It offers many of the features and capabilities of the publisher's high-end science software, such as the ability to perform many bioinformatics analyses, including interactive restriction site analysis, creating and editing alignments, phylogenetics, integrated GenBank searches, and advanced DNA to protein translation. CLC bio's Sequence Viewer is a free tool for basic bioinformatics analysis. ![]()
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